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tb green premix ex taq ii  (TaKaRa)


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    Structured Review

    TaKaRa tb green premix ex taq ii
    Tb Green Premix Ex Taq Ii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 50598 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tb+green/TB+Green+Premix+Ex+Taq+II/pmc13679839-238-5-12
    Average 99 stars, based on 50598 article reviews
    tb green premix ex taq ii - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Inhibition of Adenosine Kinase Alleviates Hypertrophic Cardiomyopathy by Ameliorating Coronary Microvascular Dysfunction.
    Article Snippet: .. Reverse transcription was performed using a kit (RR047A, TaKaRa 1 Bio, Japan) and qPCR was performed using TB-Green (Takara, RR820A) with the primers listed in Table 2 S3. ..

    Article Title: Prophylactic and/or therapeutic agent for chronic prostatitis/chronic pelvic pain syndrome
    Article Snippet: 500 ng of RNA was subjected to reverse transcription using ReverTra Ace (registered trademark) qPCR RT Master Mix (Cat. No. FSQ-201, manufactured by TOYOBO CO., LTD.) and Biometra Tadvanced 96SG (manufactured by BM Equipment Co., Ltd.). .. The cDNA obtained by reverse transcription, TB Green (registered trademark) Premix Ex Taq II (Cat. No. RR820, manufactured by Takara Bio Inc.), and LightCycler (registered trademark) 480 real-time PCR system (manufactured by Roche Diagnostics GmbH) were used to evaluate the gene expression level of CCL2. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Inhibition of Adenosine Kinase Alleviates Hypertrophic Cardiomyopathy by Ameliorating Coronary Microvascular Dysfunction.
    Article Snippet: .. Reverse transcription was performed using a kit (RR047A, TaKaRa 1 Bio, Japan) and qPCR was performed using TB-Green (Takara, RR820A) with the primers listed in Table 2 S3. ..

    Article Title: A scalable screening platform for discovering predominant miRNA target genes and druggable siRNA sequences
    Article Snippet: Maxima H Minus Reverse Transcriptase (Thermo) and its buffer were added to the samples and incubated at 53°C for 1 hr, followed by 85°C for 5 min. TaqMan qPCR was carried out using Premix Ex Taq (Probe qPCR) (Takara, cat# RR390A) in the presence of 0.4 μM TaqMan probe. .. Since the passenger sequences from shRNAmir and shRNAone were different, we included chemically synthesized 22-nt RNAs as standards. qPCR was performed using TB Green (Takara, cat# RR420A), and the absolute amounts of guide and passenger strands were quantified based on a standard curve obtained from 20 fmole, 2 fmole, and 0.2 fmole of synthetic RNA. .. In dual luciferase assays, small RNA targeting sequences were inserted into the 3’ UTR of Firefly luciferase, where inserts were prepared by primer annealing and were cloned into pmirGLO (Promega, cat# E1330) with NheI and SalI restriction sites using T4 DNA ligase (TaKaRa).

    Article Title: Engineered CXCR3-A expression enhances B7-H3-targeting CAR T cell migration and efficacy against diffuse intrinsic pontine glioma.
    Article Snippet: The RNA was extracted from cells using RNeasy Plus Mini Kit (QIAGEN, #74134) and converted to cDNA with High-Capacity RNA-to-cDNA kit (Applied Biosystems, #4387406). .. The cDNA was then set up with TB Green (Takara, #RR42LR) and a primer pair (Integrated DNA Technologies) in four replicate wells for qPCR with Light Cycler 480 II (Roche). ..

    Article Title: Prophylactic and/or therapeutic agent for chronic prostatitis/chronic pelvic pain syndrome
    Article Snippet: 500 ng of RNA was subjected to reverse transcription using ReverTra Ace (registered trademark) qPCR RT Master Mix (Cat. No. FSQ-201, manufactured by TOYOBO CO., LTD.) and Biometra Tadvanced 96SG (manufactured by BM Equipment Co., Ltd.). .. The cDNA obtained by reverse transcription, TB Green (registered trademark) Premix Ex Taq II (Cat. No. RR820, manufactured by Takara Bio Inc.), and LightCycler (registered trademark) 480 real-time PCR system (manufactured by Roche Diagnostics GmbH) were used to evaluate the gene expression level of CCL2. ..

    Article Title: Engineered CXCR3-A expression enhances B7-H3-targeting CAR T cell migration and efficacy against diffuse intrinsic pontine glioma
    Article Snippet: The RNA was extracted from cells using RNeasy Plus Mini Kit (QIAGEN, #74134) and converted to cDNA with High-Capacity RNA-to-cDNA kit (Applied Biosystems, #4387406). .. The cDNA was then set up with TB Green (Takara, #RR42LR) and a primer pair (Integrated DNA Technologies) in four replicate wells for qPCR with Light Cycler 480 II (Roche). ..

    Synthesized:

    Article Title: A scalable screening platform for discovering predominant miRNA target genes and druggable siRNA sequences
    Article Snippet: Maxima H Minus Reverse Transcriptase (Thermo) and its buffer were added to the samples and incubated at 53°C for 1 hr, followed by 85°C for 5 min. TaqMan qPCR was carried out using Premix Ex Taq (Probe qPCR) (Takara, cat# RR390A) in the presence of 0.4 μM TaqMan probe. .. Since the passenger sequences from shRNAmir and shRNAone were different, we included chemically synthesized 22-nt RNAs as standards. qPCR was performed using TB Green (Takara, cat# RR420A), and the absolute amounts of guide and passenger strands were quantified based on a standard curve obtained from 20 fmole, 2 fmole, and 0.2 fmole of synthetic RNA. .. In dual luciferase assays, small RNA targeting sequences were inserted into the 3’ UTR of Firefly luciferase, where inserts were prepared by primer annealing and were cloned into pmirGLO (Promega, cat# E1330) with NheI and SalI restriction sites using T4 DNA ligase (TaKaRa).

    other:

    Article Title: Estrogen Receptor-α36 Mediates EGFR-SGK1 Signaling-Related Erk Activation in Gastric Cancer.
    Article Snippet: One microgram of RNA was reverse-transcribed into cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (Takara, 6210A, Kusatsu, Japan).

    Gene Expression:

    Article Title: Prophylactic and/or therapeutic agent for chronic prostatitis/chronic pelvic pain syndrome
    Article Snippet: 500 ng of RNA was subjected to reverse transcription using ReverTra Ace (registered trademark) qPCR RT Master Mix (Cat. No. FSQ-201, manufactured by TOYOBO CO., LTD.) and Biometra Tadvanced 96SG (manufactured by BM Equipment Co., Ltd.). .. The cDNA obtained by reverse transcription, TB Green (registered trademark) Premix Ex Taq II (Cat. No. RR820, manufactured by Takara Bio Inc.), and LightCycler (registered trademark) 480 real-time PCR system (manufactured by Roche Diagnostics GmbH) were used to evaluate the gene expression level of CCL2. ..



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